Detection and quantification of various phenolic compounds present in the extracts was done using reverse-phase HPLC technique. In this experiment, Waters HPLC (Model 2487) instrument with a UV spectrophotometer detector was used. The column was a 15 cm hypersil C18 reverse phase column with 5μ particle packing. The mobile phase (Composition – 20% methanol, 1% acetic acid and 79% water) was passed through the column at the rate of 1ml/min. Five phenolic standards namely Gallic acid, Catechol, p-Coumaric acid, Ferulic acid and Caffeic acid were used for their detection and quantification in the plant extracts. Each phenolic standard stock was prepared (1mg/ml) in HPLC grade methanol and stored at 0°C till further use. All five phenolic standards were mixed freshly for analysis, to make 100ppm strength for each standard. This phenolic mix was injected as a single injection to obtain standard chromatogram. Run time was 50 minutes. For quantification of phenolic compounds in individual samples, plant extracts were first diluted to 100 mg/ml. All the extracts were then filtered through μm pore size cellulose syringe filters (Sigma-Aldrich). Injection volume for each sample as well as standard was 20 μl. A linear gradient elution scheme was used in this method and detection was done at 280 nm. The phenolic compounds were detected on the basis of retention time as identified by a standard chromatogram of a mixture of the pure phenolic compounds obtained beforehand. The concentration of individual phenolic compound was estimated from the peak area measurements in comparison to standards and the output was given in the units of ppm using Empower software. The results were then converted from ppm to μg/g. All the solvents and chemicals used were of HPLC grade.